#Fields: date time s-ip cs-method cs-uri-stem cs-uri-query s-port cs-username c-ip cs(User-Agent) sc-status sc-substatus sc-win32-status time-taken #Software: Microsoft Internet Information Services 7.5 PHP.INI is configured to write error log to syslog but there's nothing appearing in Syslog that tells me anything useful.
It would be helpful if I knew what the "resource looking for" but beyond telling me "There is a problem with the resource you are looking for, and it cannot be displayed"
#Vb5 ms agent upgrade
Refreshing screen/re-running upgrade script starts where it left off but produces same issue.
#Vb5 ms agent full
As this is a test rig in my man cave I've given every users full control over the VB5forum directory, but still no luck in getting upgrade to move beyond step 128. Ĭlearly a server error, and I'm thinking permissions based but I can't be sure. There is a problem with the resource you are looking for, and it cannot be displayed. Server Error 500 - Internal server error. These modification strategies were proved effective and the constructed β-alanine producer was a promising microbial cell factory for industrial production of β-alanine.500 - Internal server error. The resultant strain ALA17/pTrc99a- panD BS- aspB CG could yield 4.20 g/L β-alanine in shake flask and 43.94 g/L β-alanine (a yield of 0.20 g/g glucose) in 5-L bioreactor via fed-batch cultivation. On this basis, a plasmid harboring the heterologous panD and aspB was constructed.
Specifically, heterologous L-aspartate-α-decarboxylase was used, the aspartate kinase I and III involved in competitive pathways were down-regulated, the β-alanine uptake system was disrupted, the phosphoenolpyruvate carboxylase was overexpressed, and the isocitrate lyase repressor repressing glyoxylate cycle shunt was delete, the glucose uptake system was modified, and the regeneration of amino donor was up-regulated. In this study, metabolic design strategies were attempted in Escherichia coli W3110 for enhancing β-alanine biosynthesis. Β-Alanine is the only naturally occurring β-amino acid, widely used in the fine chemical and pharmaceutical fields.
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